A Novel Fluorometric Ultramicro Determination of Serum Leucine Aminopeptidase Using A Coumarine Derivative
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منابع مشابه
Investigation of the use of calcein in the ultramicro fluorometric determination of calcium in serum.
Calcium has been determined fluorometrically in serum with use of calcein (fluorescein-methylene-iminodiacetic acid, C30H26N203) throughout the last decade. Wallach et al. (1, 2) developed the first useable method in 1959, which was later modified by Kepner and Hercules (3) in 1963, Phillips (4) in 1964, and Hill (5) in 1965. Moser and Gerarde (6) adapted the method to the “trNOPETTE”1 System i...
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A new method to assay the enzyme, leucine aminopeptidase (LAP; L-leucyl-peptide hydrolase.-3.4.1.1), is described. The technic is an extension of the conventional glutamic-pyruvic transaminase (GPT) reaction. Serum LAP is allowed to react with the substrate L-leucyl-L-alanine to quantitatively generate alanine. This alanine, in the presence of excess -ketoglutaric acid and GPT, rapidly forms py...
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Serum leucine aminopeptidase determination was found to be a useful screening procedure for hepatobiliary disease in jaundiced and unjaundiced patients. Values under 1,000 units are of no help in the differential diagnosis of jaundice but values above 1,000 units are highly indicative of biliary obstruction. The differentiation of intra- from extrahepatic obstruction as well as of malignant fro...
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The fluorometric method for measuring leucine aminopeptidase (EC 3.4.11.1) activity in biological systems, with L-leucyl--naphthylamide as the substrate [C/in. Chem. 16, 412 (1970)] was critically evaluated and refined. In this enzyme assay system, a few precautions are necessary in determining /3-naphthylamine liberated from the substrate by the enzyme action. In water, tris(hydroxymethyl)amin...
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We investigated a family in which some individuals showed extremely high serum leucine aminopeptidase (LAP) (EC 3.4.11.2) activity, mainly derived from a variant CD13. The isoelectric points of the variant and normal CD13 were 3.3 and 4.1, respectively, and both points converged at 4.4 after treatment with neuraminidase, indicating that more sialic acids are bound to the variant CD13 than norma...
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ژورنال
عنوان ژورنال: Juntendo Medical Journal
سال: 1978
ISSN: 0022-6769,2188-2134
DOI: 10.14789/pjmj.24.469